Anti-Rab1b antibody [G2-A12]

Anti-Rab1b antibody [G2-A12]

• 概述

• 产品描述The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
• 产品名称Anti-Rab1b antibody [G2-A12]
• 分子量22 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Membrane. Cytoplasm.
• 其它名称
  • RAB1B antibody
  • RAB1B member RAS oncogene family antibody
  • RAB1B_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Rab1b on human Rab1b recombinant protein using anti-Rab1b antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Rab1b on HEK293 (1) and Rab1b-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab1b antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-Rab1b antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-Rab1b antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with Rab1b antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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