Anti-PTPN14 antibody [H7-B3]

Anti-PTPN14 antibody [H7-B3]

• 概述

• 产品描述Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.
• 产品名称Anti-PTPN14 antibody [H7-B3]
• 分子量135 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Cytoskeleton.
• 其它名称
  • Cytoskeletal associated protein tyrosine phosphatase antibody
  • MGC126803 antibody
  • PEZ antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PTPN14 on human PTPN14 ecombinant protein using anti-PTPN14 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PTPN14 on HEK293 (1) and PTPN14-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPN14 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PTPN14 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with PTPN14 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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