Anti-WASL antibody

Anti-WASL antibody

• 概述

• 产品描述Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis.

• 产品名称Anti-WASL antibody

• 分子量55 kDa (Predicted band size)

• 种属反应性Human,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Recombinant protein within human WASL aa 100-300.

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Protein affinity purified.

• 亚细胞定位Cytoplasm. Cytoskeleton. Nucleus.

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• ​其它名称WASP family, verprolin homology domain-containing protein 1 antibody
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  Verprolin homology domain containing protein 1 antibody
  Verprolin homology domain-containing protein 1 antibody
  WAS protein family, member 1 antibody
  Wasf1 antibody
  WASF1_HUMAN antibody
  WASL antibody
  WASP family 1 antibody
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  WAVE antibody
  WAVE1 antibody
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  Wiskott-Aldrich syndrome protein family member 1 antibody

  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50
  FC: 1:50-1:100

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  Fig1: Western blot analysis of WASL on SK-BR-3 and SH-SY-5Y cell lysate using anti- WASL antibody at 1/500 dilution.

   

  

  Fig2: ICC staining WASL in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining WASL in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- WASL antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti- WASL antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of SH-SY5Y cells with WASL antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the se

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