Anti-Factor D antibody

Anti-Factor D antibody

• 概述

• 产品描述Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
• 产品名称Anti-Factor D antibody
• 分子量24 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human Factor D/Adipsin 21-120/253
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Secreted.
• 其它名称adipsin
  Adipsin/complement factor D
  adn
  C3 convertase activator
  CFAD_HUMAN
  CFD
  Complement factor D
  complement factor D preproprotein
  D component of complement
  DF
  FactorD
  PFD
  Properdin factor D.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Protein:
  A549(human)cell lysates at 40ug;
  spleen disease in mouse at 40ug ;
  Primary: Anti-Factor D at 1:300;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
  ECL excitated the fluorescence;
  Predicted band size :24 kD
  Observed band size :28/75 kD

   

  

  Fig2: Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Factor D Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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