Anti-CSF3 antibody [7F-4E]

Anti-CSF3 antibody [7F-4E]

• 概述

• 产品描述Granulocyte-colony stimulating factor, G-CSF, is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The murine G-CSF cDNA encodes a 208 amino acid precursor containing a 30 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF cDNAs, which are identical except for a three amino acid deletion in the N-terminus of one form of the protein, have been isolated from human cells. Murine and human G-CSF share 73% sequence identity at the amino acid level. G-CSF signals through the G-CSF receptor, G-CSFR, a heavily glycosylated 812 amino acid polypeptide with a single transmembrane domain. Stimulation of the G-CSFR results in the activation of the Ras/MAPK pathway and phosphorylation of the adaptor protein Shc. Studies indicate that the kinases Lyn and Syk and the transcription factor Stat3 are activated in response to G-CSF stimulation.
• 产品名称Anti-CSF3 antibody [7F-4E]
• 分子量22 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Secreted.
• 其它名称
  • C17orf33 antibody
  • Colony stimulating factor 3 (granulocyte) antibody
  • Colony stimulating factor 3 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of G-CSF on human G-CSF recombinant protein using anti- G-CSF antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of G-CSF on HEK293 (1) and G-CSF-hIgGFc transfected HEK293 (2) cell lysate using anti-G-CSF antibody at 1/1,000

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- G-CSF antibody. Counter stained with hematoxylin.

   

  

  Fig4: ICC staining G-CSF (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: ICC staining G-CSF (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS

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