Anti-NME1 antibody [C6-B2]

Anti-NME1 antibody [C6-B2]

• 概述

• 产品描述The nm23 protein is implicated in a variety of biological processes, including suppressing metastasis, phytochrome responses in plants and regulating differentiation. Nm23-H1, nm23-H2 and nm23-H2, members of the nm23 family, are putative metastasis suppressor genes that encode nucleoside diphosphate kinase (NDPK) A and B. NDPKs form oligomers, which are distributed in both the soluble and particulate fractions of cells. Upon cAMP-induced differentiation of rat cells, nm23 proteins show a differential interaction with intermediate filaments. Both isoforms also associate with GFAP in differentiated cells. nm23 proteins and their mutants are localized predominantly in the mitochondria. Nm23 mutants are unable to inhibit differentiation and promote apoptosis as a results of defective protein-protein interactions in the mitochondria. Nm23-H1, nm23-H2 and nm23-H3 are indicators of a poor prognosis in human hematopoietic malignancies, and as a high expression of nm23-H1 and -H2 is positively correlated with histological

• 产品名称Anti-NME1 antibody [C6-B2]

• 分子量17 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cytoplasm. Nucleus. 

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• 其它名称

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• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

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  Fig1: Western blot analysis of NME1 on NME1-hIgGFc transfected HEK293 (2) cell lysate using anti-NME1 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining NME1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NME1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Jurkat cells with NME1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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