Anti-PLCG2 antibody [B6-A8]

Anti-PLCG2 antibody [B6-A8]

• 概述

• 产品描述Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC γ2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR γ chain), which bind to its C-terminal SH2 domain. PLC γ1 associates with Syk in B cells, but PLC γ2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC γ2. In addition, Btk can induce PLC γ2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes.
• 产品名称Anti-PLCG2 antibody [B6-A8]
• 分子量148 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol
• 其它名称
  • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
  • 1-phosphatidylinositol-4 antibody
  • 5-bisphosphate phosphodiesterase gamma-2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PLCG2 on human PLCG2 recombinant protein using anti-PLCG2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PLCG2 on HEK293 (1) and PLCG2-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PLCG2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of Hela cells with PLCG2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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