Anti-UHRF1 antibody [B1-A1]

Anti-UHRF1 antibody [B1-A1]

• 概述

• 产品描述UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1), also known as Np95 (nuclear zinc finger protein 95), ICBP90 (inverted CCAAT box-binding protein of 90 kDa) or RNF106, is a transcription and cell cycle regulator belonging to the RING-finger type E3 ubiquitin ligase subfamily. UHRF1 is expressed in bone marrow, thymus, heart, testis and lung, and contains one PHD-type zinc finger, a ubiquitin-like domain, two RING-type zinc fingers and one YDG/SRA domain. Localizing to the nucleus, UHRF1 is believed to function as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation. By mediating ubiquitination, UHRF1 plays an important role in cellular proliferation. In addition, UHRF1 directly interacts with Dnmt1 (a maintenance DNA methyltransferase) and is required for the stable association of Dnmt1 with chromatin. UHRF1 is overexpressed in cancer cells, suggesting a possible role in carcinogenesis.
• 产品名称Anti-UHRF1 antibody [B1-A1]
• 分子量90 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus
• 其它名称
  • Ac2-121 antibody
  • AL022808 antibody
  • E3 ubiquitin-protein ligase UHRF1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of UHRF1 on human UHRF1 recombinant protein using anti-UHRF1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of UHRF1 on HEK293 (1) and UHRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-UHRF1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of UHRF1 on MCF-7 (1) and Hela (2) cell lysate using anti-UHRF1 antibody at 1/1,000 dilution.

   

  

  Fig4: ICC staining UHRF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-UHRF1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using anti-UHRF1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of MCF-7 cells with UHRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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