Anti-Dopamine D5 receptor antibody
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概述
- 产品名称Anti-Dopamine D5 receptor antibody
- 分子量53 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from rat DRD5 251-350/475
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein
- 其它名称D(1B) dopamine receptor
D(5) dopamine receptor
D1 beta dopamine receptor
D1B dopamine receptor
D1beta dopamine receptor
D5 dopamine receptor
DBDR
Dopamine receptor D1B
DRD 1B
DRD 5
DRD1B
DRD1L2
DRD5
MGC10601
DRD5_HUMAN
DRD5_RAT.
more
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应用
WB:1:500-2000
FC:1μg /test
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Fig1: Blank control: RSC96(blue).
Primary Antibody:Rabbit Anti- DRD5 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Antibody (ER1907-73, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Fig2: Sample:
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D5 receptor (ER1907-73)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53kD
Observed band size: 53kD
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