Anti-IRAK3 antibody [G2-G9]

Anti-IRAK3 antibody [G2-G9]

• 概述

• 产品描述Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkB signaling pathway. An IL-1-dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFkB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFkB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1.
• 产品名称Anti-IRAK3 antibody [G2-G9]
• 分子量68 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, nucleus
• 其它名称
  • ASRT5 antibody
  • IL 1 receptor associated kinase M antibody
  • IL-1 receptor-associated kinase M antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of IRAKM on human IRAKM recombinant protein using anti- IRAKM antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of IRAKM on HEK293 (1) and IRAKM -hIgGFc transfected HEK293 (2) cell lysate using anti-IRAKM antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining IRAKM (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining IRAKM (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- IRAKM antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of HepG2 cells with IRAKM antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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