Anti-PUMA antibody

Anti-PUMA antibody

• 概述

• 产品描述The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis.

• 产品名称Anti-PUMA antibody

• 分子量20 kDa

• 种属反应性Human,Mouse

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Mitochondrion

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• 其它名称BBC 3 antibody
  Bbc3 antibody
  BBC3_HUMAN antibody
  BCL 2 binding component 3 antibody
  Bcl-2-binding component 3 antibody
  BCL2 binding component 3 antibody
  JFY 1 antibody
  JFY-1 antibody
  JFY1 antibody
  p53 up regulated modulator of apoptosis antibody
  p53 up-regulated modulator of apoptosis antibody
  p53 Upregulated Modulator of Apoptosis antibody
  PUMA alpha antibody
  PUMA/JFY1 antibody

  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC:1:100

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  Fig1: Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: Mouse kidney

   

  

  Fig2: ICC staining PUMA in SKOV-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining PUMA in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining PUMA in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PUMA antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Jurkat cells with PUMA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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