Anti-CAV2 antibody [2E-9E]

Anti-CAV2 antibody [2E-9E]

• 概述

• 产品描述Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae (6-8). Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms (6-8)
• 产品名称Anti-CAV2 antibody [2E-9E]
• 分子量18 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane.
• 其它名称
  • CAV antibody
  • CAV2 antibody
  • CAV2_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of caveolin-2 on human caveolin-2 recombinant protein using anti- caveolin-2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of caveolin-2 on HEK293 (1) and caveolin-2-hIgGFc transfected HEK293 (2) cell lysate using anti- caveolin-2

   

  

  Fig3: Western blot analysis of caveolin-2 on different cell lysate using anti- caveolin-2 antibody at 1/1,000 dilution.
  Positive control: Line1: A549 Line2: 3T3-L1 Line3: A431

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- caveolin-2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- caveolin-2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of A549 cells with caveolin-2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！