Anti-RBM20 antibody

Anti-RBM20 antibody

• 概述

• 产品描述RNA-binding protein that acts as a regulator of mRNA splicing of a subset of genes involved in cardiac development. Regulates splicing of TTN (Titin).
• 产品名称Anti-RBM20 antibody
• 分子量134 KDa
• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Sheep
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human RBM20 361-460/1227
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nucleus (By similarity).
• 其它名称Probable RNA-binding protein 20
  Rbm20
  RBM20_HUMAN
  RNA-binding motif protein 20.

• 应用

  WB:1:500-2000
  FC:3ug/test

•  

  

  Fig1: Sample: heart (mouse) Lysate at 40 ug
  Primary: Anti- RBM20at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 75kD
  Observed band size: 75 kD

   

  

  Fig2: Sample:
  K562(Human) Cell Lysate at 30 ug
  A549(Human) Cell Lysate at 30 ug
  Primary: Anti- RBM20 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 134 kD
  Observed band size: 130 kD

   

  

  Fig3: Blank control:A549.
  Primary Antibody (green line): Rabbit Anti-RBM20 antibody (
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 3μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！