Anti-CELF-1 antibody [7A1]

Anti-CELF-1 antibody [7A1]

• 概述

• 产品描述Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawl.
• 产品名称Anti-CELF-1 antibody [7A1]
• 分子量52 kDa
• 种属反应性Human
• 验证应用IHC-P,WB
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human CELF1 aa 1-180.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein affinity purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • 50 kDa Nuclear polyadenylated RNA binding protein antibody
  • 50 kDa nuclear polyadenylated RNA-binding protein antibody
  • Bruno like 2 antibody
  more

• 应用

  IHC-P: 1:50-1:200
  WB: 1:2,000-1:5,000

•  

  

  Fig1: Western blot analysis of CELF1 on Daudi cell lysate using anti-CELF1 antibody at 1/5,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CELF1 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CELF1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CELF1 antibody. Counter stained with hematoxylin.

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