Anti-Atg12 antibody
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概述
- 产品描述Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.
- 产品名称Anti-Atg12 antibody
- 分子量8/15 kDa
- 种属反应性Human
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within human Atg12 aa 1-100.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm. Membrane.
- 其它名称APG12-like antibody
APG12L antibody
ATG12 antibody
ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
ATG12 autophagy related 12 homolog antibody
ATG12_HUMAN antibody
Autophagy 12 antibody
Autophagy-related protein 12 antibody
FBR93 antibody
HAPG12 antibody
Ubiquitin-like protein ATG12 antibody
more
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应用
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Human kidney tissue
Lane 3: 293T
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
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