Anti-Histone H3 antibody [A11-D7]

Anti-Histone H3 antibody [A11-D7]

• 概述

• 产品描述The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• 产品名称Anti-Histone H3 antibody [A11-D7]
• 分子量17 kDa
• 种属反应性Human,Mouse,Rat, Zebrafish
• 验证应用WB, ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus

• 应用

  WB: 1:5,000-1:10,000
  ICC: 1:200-1:500
  IHC-P: 1:200-1:1000

•  

  

  Fig1: Western blot analysis of Histone H3 on different cell lysates using anti-Histone H3 antibody at 1/2,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: PC-12
  Lane 3: F9

   

  

  Fig2: Immunofluorescent staining of Hela cells using anti-Histone H3 Mouse mAb.

   

  

  Fig3: Immunofluorescent staining of F9 cells using anti-Histone H3 Mouse mAb.

   

  

  Fig4: Western blot analysis of Histone H3 on hybrid fish (crucian-carp) brain tissue lysate using anti-Histone H3 antibody at 1/500 dilution.

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