Anti-PBK antibody [E11-C8]

Anti-PBK antibody [E11-C8]

• 概述

• 产品描述Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Kinases are capable of influencing the oncogenic potential of cell sytems at the level of oncoprotein or tumor suppressor protein phosphorylation states. Human PDZ-binding kinase, known as PBK, is a 322 amino acid, T/SXV motif-containing serine/threonine kinase that is abuntant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. The cell cycle checkpoint kinase Cdc2/cyclin B is an upstream effector of PBK that can phosphorylate and activate PBK. Active PBK may associate with PDZ-containing proteins and influence cell cycle control or cellular proliferation.
• 产品名称Anti-PBK antibody [E11-C8]
• 分子量36 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • Cancer/testis antigen 84 antibody
  • CT84 antibody
  • Epididymis luminal protein 164 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PBK on human PBK recombinant protein using anti-PBK antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PBK on A431 cell lysate using anti-PBK antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PBK (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBK antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with PBK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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