Anti-CEBPA antibody [E8-B7]

Anti-CEBPA antibody [E8-B7]

• 概述

• 产品描述The transcription factor C/EBP α (CCAAT-enhancer binding protein) is a heat-stable, sequence-specific DNA-binding protein that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growth-arrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA binding activity of C/EBP α.
• 产品名称Anti-CEBPA antibody [E8-B7]
• 分子量42 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • Apoptotic cysteine protease antibody
  • Apoptotic protease Mch 5 antibody
  • C/EBP alpha antibody
  more

• 应用

  WB: 1:500
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CEBP alpha on different cell lysates using anti-CEBP alpha antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: k562
  Lane 3: HepG2

   

  

  Fig2: ICC staining CEBP alpha (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human rectum tissue tissue using anti-CEBP alpha antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of MCF-7 cells with CEBP alpha antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！