Anti-Na+/K+ ATPase antibody [1G1]

Anti-Na+/K+ ATPase antibody [1G1]

• 概述

• 产品描述The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase.
• 产品名称Anti-Na+/K+ ATPase antibody [1G1]
• 分子量100-143kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 551-850 of Na+/K+-ATPase α1 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Melanosome, Cell membrane
• 其它名称
  • ATPase Na+/K+ transporting alpha antibody
  • adenosinetriphosphatase antibody
  • AT1A1_HUMAN antibody
  more

• 应用

  WB: 1:2,000-1:1,0000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

•  

  

  Fig1: Western blot analysis of Na+/K+-ATPase α expression in human kidney (A) and human brain (B) tissue extracts.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing membrane staining of glandular cells (B).

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