Anti-SARS-CoV-2 Nucleocapsid Protein antibody [A4D12]

Anti-SARS-CoV-2 Nucleocapsid Protein antibody [A4D12]

• 概述

• 产品描述Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• 产品名称Anti-SARS-CoV-2 Nucleocapsid Protein antibody [A4D12]
• 分子量46 kDa
• 种属反应性SARS-CoV-2
• 验证应用ELISA, WB
• 抗体类型小鼠单抗
• 免疫原Recombinant SARS-CoV-2 Nucleocapsid Protein.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4).
• 亚型IgG1
• 纯化方式Protein G affinity purified.
• 亚细胞定位Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus.
• 其它名称
  • SARS-CoV-2 Nucleocapsid Protein antibody
  • SARS-CoV-2 NP antibody
  • nucleocapsid protein antibody
  more

• 应用

  ELISA: Use at an assay dependent concentration.
  WB: 1:500-1:5,000

•  

  

  Fig1: The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.
  Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of21 is 271.3 ng/ml.

   

  

  Fig2: Western blot analysis of EM1902-21 with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and Inactivated SARS-CoV-2 lysate (2) at 20ng.
  Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.

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