Anti-Cyclin A2 antibody [N0-E3]

Anti-Cyclin A2 antibody [N0-E3]

• 概述

• 产品名称Anti-Cyclin A2 antibody [N0-E3]
• 分子量48 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位--

• 应用

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  Fig1: Western blot analysis on different cell lysates using anti-Cyclin A2 Mouse mAb.
  Positive control:
  Lane 1: 293
  Lane 2: F9
  Lane 3: PC12
  Lane 4: Hela

   

  

  Fig2: ICC staining Cyclin A2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Cyclin A2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Flow cytometric analysis of Hela cells with Cyclin A2 antibody at 1/100 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the second

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