Anti-PTBP1 antibody [9A1]

Anti-PTBP1 antibody [9A1]

• 概述

• 产品描述Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
• 产品名称Anti-PTBP1 antibody [9A1]
• 分子量57 kDa
• 种属反应性Human,Rat
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within human PTBP1 aa 320-550.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein G purified.
• 亚细胞定位Nucleus.
• 其它名称
  • 57 kDa RNA binding protein PPTB 1 antibody
  • 57 kDa RNA-binding protein PPTB-1 antibody
  • Heterogeneous nuclear ribonucleoprotein I antibody
  more

• 应用

  WB: 1:5,000-1:10,000
  IHC-P: 1:50-1:100
  ICC: 1:50-1:100
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PTBP1 on different cell lysates using anti-PTBP1 antibody at 1/5,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: A431
  Lane 3: K562

   

  

  Fig2: ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin.

   

  

  Fig9: Flow cytometric analysis of K562 cells with PTBP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary

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