Anti-Tyrosine Hydroxylase antibody
-
概述
- 产品描述The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson's disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds.
- 产品名称Anti-Tyrosine Hydroxylase antibody
- 分子量45/58 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
more
-
应用
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of Tyrosine Hydroxylase on PC-12 cell lysate using anti-Tyrosine Hydroxylase antibody at 1/1,000 dilution.
Fig2: Western blot analysis of Tyrosine Hydroxylase on mouse brain tissue (1) and MCF-7 cell (2) lysate using anti-Tyrosine Hydroxylase antibody at 1/500 dilution.
Fig3: ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Tyrosine Hydroxylase in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY5Y cells with Tyrosine Hydroxylase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
