Anti-Granzyme B antibody [4G9]

Anti-Granzyme B antibody [4G9]

• 概述

• 产品描述Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.
• 产品名称Anti-Granzyme B antibody [4G9]
• 分子量32 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 1-247 representing full length granzyme B of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasmic granule
• 其它名称
  • C11 antibody
  • Cathepsin G like 1 antibody
  • Cathepsin G-like 1 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-500
  IHC-P: 1:50-500

•  

  

  Fig1: Western blot analysis of granzyme B expression in non-transfected(A)and human granzyme B transfected(B)293T whole cell lysates.

   

  

  Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.

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