Anti-5HT2A Receptor antibody
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概述
- 产品描述This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.
- 产品名称Anti-5HT2A Receptor antibody
- 分子量52 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,ICC/IF,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human 5HT2A Receptor 65-160/471
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses.
- 其它名称5-hydroxytryptamine receptor 2A
5-hydroxytryptamine receptor
Serotonin receptor 2A
5-HT-2A
5-HT-2
HTR2A
Htr2a
5 HT 2
5HTR2A
5 HT 2A
5 HT2 receptor
5 HT2A
5 hydroxytryptamine receptor 2A
5-HT-2
5-HT-2A
5-hydroxytryptamine 2A receptor
5-hydroxytryptamine receptor 2A
5HT2A_HUMAN
HTR 2
HTR 2A
HTR2
HTR2A.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:3ug/Test
IF:1:200-800
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Fig1: Sample:
U251(Human) Cell Lysate at 30 ug
BV2(Mouse) Cell Lysate at 30 ug
Primary: Anti-5HT2A Receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Fig2: Blank control: U-2OS.
Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig5: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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