Anti-EGFR antibody

Anti-EGFR antibody

• 概述

• 产品描述The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Exons in the EGFR gene product are frequently either deleted or duplicated to produce deletion mutants (DM) or tandem duplication mutants (TDM), respectively, which are detected at various molecular weights. EGFR binds several ligands, including epidermal growth factor (EGF), transforming growth factor α (TGFα), Amphiregulin and heparin binding-EGF (HB-EGF). Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target.
• 产品名称Anti-EGFR antibody
• 分子量175 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Recombinant protein.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein A purified.
• 亚细胞定位Secreted and Cell membrane. Endosome membrane. Nucleus.
• 其它名称
  • Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
  • Cell growth inhibiting protein 40 antibody
  • Cell proliferation inducing protein 61 antibody
  more

• 应用

  WB: 1:500-1:2000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of EGFR on A431 (1) and HepG2 (2) cell lysate using anti-EGFR antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining EGFR in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining EGFR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-EGFR antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-EGFR antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-EGFR antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-EGFR antibody. Counter stained with hematoxylin.

   

  

  Fig8: Flow cytometric analysis of PANC-1 cells with EGFR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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