Anti-Carbonic anhydrase 2 antibody [11A2]

Anti-Carbonic anhydrase 2 antibody [11A2]

• 概述

• 产品描述Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes including respiration, calcification, acid-base balance and bone resorption, as well as the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA2 gene, which maps to chromosome 8q21, encodes CA II, a cytoplasmic protein that has the highest turnover rate and widest tissue distribution of any known human CA isozyme. The human CA4 gene, which maps to chromosome 17q23, encodes CA IV, a membrane-anchored isozyme that is expressed on the luminal surfaces of pulmonary capillaries and proximal renal tubules. The human CA9, CA12 and CA14 genes, which map to chromosomes 9p13, 15q22 and 1q21, respectively, encode transmembrane proteins that have unique patterns of tissue-specific expression.
• 产品名称Anti-Carbonic anhydrase 2 antibody [11A2]
• 分子量29 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within Human Carbonic anhydrase 2 aa 50-220.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/ml.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein affinity purified.
• 亚细胞定位Plasma membrane, Cytoplasm.
• 其它名称
  • can antibody
  • CAN_ECOLI antibody
  • Carbonate dehydratase 2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC:1:50-1:200
  IHC-P:1:50-1:200

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  Fig1: Western blot analysis of Carbonic anhydrase 2 on different cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: THP-1 cell lysate
  Lane 2: HL-60 cell lysate

   

  

  Fig2: ICC staining Carbonic anhydrase 2 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig3: ICC staining Carbonic anhydrase 2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carbonic anhydrase 2 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Carbonic anhydrase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Carbonic anhydrase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibodyat 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Carbonic anhydrase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Carbonic anhydrase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues w

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Carbonic anhydrase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were block

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