Anti-N-Cadherin antibody
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概述
- 产品描述The cadherins are a large family of cell adhesion molecules involved in calcium-dependent recognition and adhesion events. The cadherin subfamily includes N-, P-, B-, and E- cadherins, and other members. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. N-Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. When a cancer cell adheres to the endothelial cells of a blood vessel it up-regulates the src kinase pathway, which phosphorylates beta-catenins attached to both N-cadherin (this protein) and E-cadherins.
- 产品名称Anti-N-Cadherin antibody
- 分子量140 kDa
- 种属反应性Human, Mouse
- 验证应用WB, ICC, IHC-P, FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide (KLH-coupled) within human N-cadherin 360-420 aa.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cell membrane
- 其它名称CADH2_HUMAN antibody
Cadherin 2 antibody
Cadherin 2 N cadherin neuronal antibody
Cadherin 2 type 1 antibody
Cadherin 2 type 1 N cadherin neuronal antibody
Cadherin 2, type 1, N-cadherin (neuronal) antibody
Cadherin-2 antibody
Cadherin2 antibody
Calcium dependent adhesion protein neuronal antibody
CD325 antibody
CD325 antigen antibody
CDH2 antibody
CDHN antibody
CDw325 antibody
CDw325 antigen antibody
N cadherin 1 antibody
N-cadherin antibody
NCAD antibody
Neural cadherin antibody
OTTHUMP00000066304 antibody
OTTHUMP00000067378 antibody
more
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应用
WB: 1:500
ICC: 1:200
IHC-P: 1:200
FC: 1:50
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Fig1: Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart lysates
Lane2 : Human heart lysates
Fig2: ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
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