Anti-ULK2 antibody

Anti-ULK2 antibody 

• 概述

• 产品描述ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species-conserved carboxyl-terminal domain. Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively. ULK1 maps to human chromosome 12q24.33 and is ubiquitously expressed. ULK2, also widely expressed, maps to mouse chromosome 11 B2 and is expected to have a similar molecular weight as ULK1 in human. ULK1 and ULK2 are thought to autophosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes.
• 产品名称Anti-ULK2 antibody
• 分子量113 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasmic vesicle membrane. Peripheral membrane protein.
• 其它名称
  • ATG1B antibody
  • KIAA0623 antibody
  • Serine/threonine protein kinase ULK2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of ULK2 on human ULK2 recombinant protein using anti-ULK2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ULK2 on HEK293 (1) and ULK2-hIgGFc transfected HEK293 (2) cell lysate using anti-ULK2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of ULK2 on different cell lysate using anti-ULK2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: NIH/3T3
  Lane 2: HepG2
  Lane 3: SK-Hep-1
  Lane 4: SK-OV-3
  Lane 5: C6
  Lane 6: PC-12
  Lane 7: MCF-7

   

  

  Fig4: Flow cytometric analysis of Hela cells with ULK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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