Anti-Ras antibody

Anti-Ras antibody

• 概述

• 产品描述Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

• 产品名称Anti-Ras antibody

• 分子量21 kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoplasmic side, cell membrane, lipid-anchor, golgi apparatus membrane

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• ​其它名称C-BAS/HAS antibody
  c-H-ras antibody
  C-HA-RAS1 antibody
  CTLO antibody
  GTPase HRas antibody
  GTPase KRas antibody
  GTPase NRas antibody
  H-Ras-1 antibody
  H-RASIDX antibody
  Ha-Ras antibody
  HAMSV antibody
  HRAS antibody
  HRAS1 antibody
  K RAS2A antibody
  K RAS2B antibody
  K RAS4A antibody
  K RAS4B antibody
  K-RAS antibody
  KRAS antibody
  KRAS1 antibody
  KRAS2 antibody
  N-RAS antibody
  N-terminally processed antibody
  NRAS antibody
  NRAS1 antibody
  p21ras antibody
  RASH_HUMAN antibody
  RASH1 antibody
  RASK2 antibody
  Transforming protein p21 antibody
  v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
  v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
  v ras neuroblastoma RAS viral oncogene homolog antibody

  more

• 应用

  WB: 1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of Ras on different cell lysates using anti-Ras antibody at 1/1000 dilution.
  Positive control:
  Lane 1: PC12
  Lane 2: MCF-7
  Lane 3: 293T
  Lane 4: Mouse brain
  Lane 5: Mouse intestine
  Lane 6: Rat brain

   

  

  Fig2: ICC staining Ras in HCT116 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Ras in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining Ras in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: ICC staining Ras in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Ras antibody. Counter stained with hematoxylin.

   

  

  Fig9: Immunohistochemical analysis of paraffin-embedded human kidney muscle tissue using anti-Ras antibody. Counter stained with hematoxylin.

   

  

  Fig10: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ras antibody. Counter stained with hematoxylin.

   

  

  Fig11: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Ras antibody. Counter stained with hematoxylin.

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