Anti-ATF6 antibody [8D3]

Anti-ATF6 antibody [8D3]

• 概述

• 产品描述Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
• 产品名称Anti-ATF6 antibody [8D3]
• 分子量75 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within full length sequence of human ATF6.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式ProG purified.
• 亚细胞定位Endoplasmic reticulum. Nucleus.
• 其它名称
  • Activating transcription factor 6 alpha antibody
  • Activating transcription factor 6 antibody
  • ATF 6 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:100
  FC:1:50-1:100

•  

  

  Fig1: Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATF6 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATF6 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of HepG2 cells with ATF6 antibody at 1/50 dilution (pink purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

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