Anti-TNNT2 antibody [5-C7]

Anti-TNNT2 antibody [5-C7]

• 概述

• 产品描述Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).Troponin T type 2 (cTnT,TNNT2) is a cardiac troponin T isoform expressed in the human heart, that is essential for calcium-regulated myofibrillar ATPase activity. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.
• 产品名称Anti-TNNT2 antibody [5-C7]
• 分子量36 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide within human TNNT2 50-100 aa.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoskeleton. Cytosol.
• 其它名称
  • Cardiac muscle troponin T antibody
  • Cardiomyopathy dilated 1D (autosomal dominant) antibody
  • Cardiomyopathy hypertrophic 2 antibody
  more

• 应用

  WB: 1:2,000-1:5,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of TNNT2 on human heart tissue lysate using anti-TNNT2 antibody at 1/2,000 dilution.

   

  

  Fig2: ICC staining TNNT2 (red) in SK-Br-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of SK-Br-3 cells with TNNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

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