Anti-NR1H4 antibody
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概述
- 产品描述This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
- 产品名称Anti-NR1H4 antibody
- 分子量56 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human FXR/Bile Acid Receptor NR1H4 175-280/486
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus.
- 其它名称Bile Acid Receptor NR1H4
BAR
FXR
Farnesoid X activated receptor
Farnesoid X receptor
Farnesoid X-activated receptor
Farnesol receptor HRR 1
Farnesol receptor HRR-1
Farnesol receptor HRR1
FXR
HRR 1
HRR1
NR1H4
NR1H4_HUMAN
Nuclear receptor subfamily 1 group H member 4
Retinoid X receptor interacting protein 14
Retinoid X receptor-interacting protein 14
RIP 14
RIP14
RXR interacting protein 14
RXR-interacting protein 14.
more
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应用
WB:1:500-2000
FC:3ug/test
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Fig1: Sample:
HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-NR1H4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig2: Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-NR1H4at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig3: Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-NR1H4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig4: Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- NR1H4 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig5: Blank control:A549.
Primary Antibody (green line): Rabbit Anti-NR1H4 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig6: Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-NR1H4at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
