Anti-KPNA2 antibody
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概述
- 产品描述Protein transport across the nucleus is a selective, multi-step process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Two cytosolic factors centrally involved in the recognition and docking process are the karyopherin a1 and karyopherin β1 subunits. Karyopherin a1 functions in the recognition and targeting of substrates destined for nuclear import, while karyopherin β1 serves as an adapter, tethering the karyopherin α1/substrate complex to docking proteins on the nuclear envelope termed nucleoporins. Karyopherin a2 has been shown to complex with Epstein-Barr virus nuclear antigen 1 (EBNA1). Certain RNA-binding proteins are imported to the nucleus by karyopherin β2, and karyopherin β3 appears to be involved in the import of some ribosomal proteins.
- 产品名称Anti-KPNA2 antibody
- 分子量58 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称
- IMA1_HUMAN antibody
- Importin alpha 1 antibody
- Importin alpha 2 antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:100-1:200
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Fig1: Western blot analysis of KPNA2 on human KPNA2 recombinant protein using anti-KPNA2 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of KPNA2 on HEK293 (1) and KPNA2-hIgGFc transfected HEK293 (2) cell lysate using anti-KPNA2 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of KPNA2 on different cell lysate using anti-KPNA2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HEK293
Lane 3: NIH/3T3
Fig4: ICC staining KPNA2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KPNA2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-KPNA2 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with KPNA2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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