Anti-Beta tubulin antibody [A1-A4]

Anti-Beta tubulin antibody [A1-A4]

• 概述

• 产品描述Tubulins is one of several members of a small family of globular proteins. The most common members of the tubulins family are α-tubulins and β-tubulins. The beta-tubulins (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulins heterodimer, it is coded by multiple tubulins genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulins may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
• 产品名称Anti-Beta tubulin antibody [A1-A4]
• 分子量52 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG3
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • beta 3 tubulin antibody
  • beta-4 antibody
  • CDCBM antibody
  more

• 应用

  WB: 1:5,000-1:10,000
  ICC: 1:200-1:500
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis on 1、NIH/3T3 2、Hela lysates using anti-β-tubulin Mouse mAb (Cat. # M1305-2).

   

  

  Fig2: Immunofluorescent staining on SKOV-3 cells using anti-β-tubulin mouse mAb.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of SHSY-5Y cells with beta tubulin antibody at 1/50 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

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