Anti-PCDH20 antibody

Anti-PCDH20 antibody

• 概述

• 产品描述Potential calcium-dependent cell-adhesion protein.

• 产品名称Anti-PCDH20 antibody

• 分子量98 KDa

• 种属反应性Human,Mouse,Rat,Horse,Rabbit

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PCDH20 611-710/924

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane.

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• ​其它名称PCD20_HUMAN
  PCDH 13
  PCDH 20
  PCDH13
  PCDH20
  Protocadherin 13
  Protocadherin 20
  Protocadherin-13
  Protocadherin-20
  Protocadherin13
  Protocadherin20.

  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

•  

  

  Fig1: Sample: MDA-MB-231 Cell (Human) Lysate at 40 ug
  Primary: Anti-PCDH20 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 98 kD
  Observed band size: 98 kD

   

  

  Fig2: Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti- PCDH20 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

   

  

  Fig3: Sample:
  MCF-7(Human) Cell Lysate at 30 ug
  Primary: Anti-PCDH20  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 98 kD
  Observed band size: 100 kD

   

  

  Fig4: Sample:
  Hippocampus (Mouse) Lysate at 40 ug
  Olfactory bulb (Mouse) Lysate at 40 ug
  Primary: Anti-PCDH20 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 98 kD
  Observed band size: 100 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！