Anti-NBN antibody [A2-E4]

Anti-NBN antibody [A2-E4]

• 概述

• 产品描述Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
• 产品名称Anti-NBN antibody [A2-E4]
• 分子量95 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Nucleus, PML body.
• 其它名称
  • AT V1 antibody
  • AT V2 antibody
  • ATV antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of p95 NBS1 on human p95 NBS1 recombinant protein using anti- p95 NBS1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of p95 NBS1 on HEK293 (1) and p95 NBS1 -hIgGFc transfected HEK293 (2) cell lysate using anti- p95 NBS1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of p95 NBS1 on different cell lysate using anti- p95 NBS1 antibody at 1/1,000 dilution.
  Positive control: Line1: A549 Line2: Jurkat Line3: PC-12

   

  

  Fig4: ICC staining p95 NBS1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- p95 NBS1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- p95 NBS1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with p95 NBS1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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