Anti-ARFGAP1 antibody [E2-C4]

Anti-ARFGAP1 antibody [E2-C4]

• 概述

• 产品描述GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
• 产品名称Anti-ARFGAP1 antibody [E2-C4]
• 分子量45 kDa
• 种属反应性Human,Mouse
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Golgi apparatus. Associates with the Golgi complex.
• 其它名称
  • ADP ribosylation factor 1 GTPase activating protein antibody
  • ADP ribosylation factor GTPase activating protein 1 antibody
  • ADP-ribosylation factor 1 GTPase-activating protein antibody
  more

• 应用

  WB: 1:500-1:1,000
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of ARFGAP1 on human ARFGAP1 recombinant protein using anti-ARFGAP1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ARFGAP1 on HEK293 (1) and ARFGAP1-hIgGFc transfected HEK293 (2) cell lysate using anti-ARFGAP1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of ARFGAP1 on different cell lysate using anti-ARFGAP1 antibody at 1/1,000 dilution.
  Positive control: Line1: MOLT4 Line2: C2C12 Line3: HepG2 Line4: MCF-7 Line5: Lncap

   

  

  Fig4: Flow cytometric analysis of HepG2 cells with ARFGAP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！