Anti-CXCR4 antibody
-
概述
- 产品描述The C-X-C or a chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 (SDF-1). SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemo-attractant for T cells and monocytes but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB and CXCR-4 (also known as LESTR or fusin). CXCR-4 is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. CXCR-4 has been identified as the major co-receptor for T-tropic HIV-1 and SDF-1 has been shown to inhibit HIV-1 infection.
- 产品名称Anti-CXCR4 antibody
- 分子量45 kDa
- 种属反应性Human,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cell junction. Plasma membrane. Endosome. Lysosome.
- 其它名称C-X-C chemokine receptor type 4 antibody
CD184 antibody
CD184 antigen antibody
Chemokine (C X C motif) receptor 4 antibody
Chemokine CXC Motif Receptor 4 antibody
CXC-R4 antibody
CXCR-4 antibody
CXCR4 antibody
CXCR4_HUMAN antibody
D2S201E antibody
FB22 antibody
Fusin antibody
HM89 antibody
HSY3RR antibody
LAP 3 antibody
LAP3 antibody
LCR1 antibody
LESTR antibody
Leukocyte derived seven transmembrane domain receptor antibody
Leukocyte-derived seven transmembrane domain receptor antibody
Lipopolysaccharide associated protein 3 antibody
Neuropeptide Y receptor Y3 antibody
NPY3R antibody
NPYR antibody
NPYRL antibody
NPYY3 antibody
NPYY3R antibody
Probable G protein coupled receptor lcr1 homolog antibody
SDF 1 receptor antibody
SDF-1 receptor antibody
SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody
Stromal cell derived factor 1 receptor antibody
Stromal cell-derived factor 1 receptor antibody
WHIM antibody
WHIMS antibody
more
-
应用
WB: 1:500-1:1,000
ICC: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of CXCR4 on different cell lysates using anti-CXCR4 antibody at 1/500 dilution.
Positive control:
Lane 1: 293
Lane 2: K562
Fig2: ICC staining CXCR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CXCR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of THP-1 cells with CXCR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondar
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
