Anti-MARK3 antibody [H12-G2]

Anti-MARK3 antibody [H12-G2]

• 概述

• 产品描述MARK3 (MAP/microtubule affinity-regulating kinase 3), also known as C-TAK1 (Cdc25C-associated protein kinase 1), EMK-2 (ELKL motif kinase 2), protein kinase STK10 or serine/threonine-protein kinase p78, is a 753 amino acid peripheral membrane protein that phosphorylates Cdc25C on Serine 216 and is ubiquitously expressed in various human tissue and cell lines. Existing as six alternatively spliced isoforms, MARK3 belongs to the Ser/Thr protein kinase family and the protein kinase superfamily. MARK3 has been suggested to mediate the binding of the 14-3-3 proteins through its kinase activity and acts as a negative regulator of mitosis. The gene encoding MARK3 maps to human chromosome 14q32.3 and mouse chromosome 12 F1.
• 产品名称Anti-MARK3 antibody [H12-G2]
• 分子量84 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane. Peripheral membrane protein.
• 其它名称
  • C-TAK1 antibody
  • Cdc25C associated protein kinase 1 antibody
  • Cdc25C-associated protein kinase 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of MARK3 on human MARK3 recombinant protein using anti-MARK3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of MARK3 on different cell lysate using anti-MARK3 antibody at 1/1,000 dilution.
  Positive control:
  Line1:HeLa Line2:SK-N-SH Line3:K562 Line4:HCT116 Line5:HEK293 Line6:3T3L1 Line7:NIH3T3 Line8:Jurkat Line9:A431

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-MARK3 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of SK-N-SH cells with MARK3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！