Anti-PRLR antibody

Anti-PRLR antibody

• 概述

• 产品描述This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

• 产品名称Anti-PRLR antibody

• 分子量67 KDa

• 种属反应性Human,Mouse,Rat,Pig,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Prolactin Receptor 251-350/622

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein. Isoform 7: Secreted.

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• ​其它名称Lactogen receptor
  MGC105486
  OPR
  Pr-1
  Pr-3
  PRL R
  PRL-R
  PRLR
  Prlr-rs1
  PRLR_HUMAN
  Prolactin receptor a
  Prolactin receptor
  Prolactin receptor delta 7/11
  RATPRLR
  Secreted prolactin binding protein
  Truncated testis-specific box 1-C prolactin receptor
  wu:fj65c07
  AI987712
  CLONE SPM213
  CPRLP
  Delta 4-delta 7/11 truncated prolactin receptor
  Delta 4-SF1b truncated prolactin receptor
  hPRL receptor
  hPRLrI.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample: Brain (Mouse) Lysate at 40 ug
  Primary: Anti-PRLRat 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 67 kD
  Observed band size: 67 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (PRLR) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-PRLR Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！