Anti-DNMT1 antibody [A2-C11]

Anti-DNMT1 antibody [A2-C11]

• 概述

• 产品描述Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
• 产品名称Anti-DNMT1 antibody [A2-C11]
• 分子量183 kDa
• 种属反应性Human,Monkey
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus
• 其它名称
  • ADCADN antibody
  • AIM antibody
  • CXXC finger protein 9 antibody
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• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of DNMT1 on human DNMT1 recombinant protein using anti-DNMT1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DNMT1 on HEK293 (1) and DNMT1-hIgGFc transfected HEK293 (2) cell lysate using anti-DNMT1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DNMT1 on different cell lysate using anti-DNMT1 antibody at 1/1,000 dilution.
  Positive control: Line1: Jurkat Line2: Cos7 Line3: HCT116 Line4: NTERA-2

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-DNMT1 antibody. Counter stained with hematoxylin.

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