Anti-PLK1 antibody [G10-C11]

Anti-PLK1 antibody [G10-C11]

• 概述

• 产品描述Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and CDC5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle and both protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation.
• 产品名称Anti-PLK1 antibody [G10-C11]
• 分子量68 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • Cell cycle regulated protein kinase antibody
  • PLK 1 antibody
  • PLK antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PLK1 on human PLK1 recombinant protein using anti-PLK1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PLK1 on K562 (1) and Raji (2) cell lysate using anti-PLK1 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PLK1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PLK1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of NIH-3T3 cells with PLK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！