Anti-Taq DNA Polymerase Antibody [3C3]

Anti-Taq DNA Polymerase Antibody [3C3]

• 概述

• 产品描述Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.
• 产品名称Anti-Taq DNA Polymerase Antibody [3C3]
• 验证应用Antibody-mediated hot start PCR
• 抗体类型小鼠单抗
• 免疫原Taq DNA Polymerase
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位--

• 应用

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  Fig1: Application of the hot start PCR using anti-Taq DNA Polymerase antibody
  Lane 1: Taq DNA Polymerase
  Lane 2: Taq DNA Polymerase + Anti-Taq DNA Polymerase antibody
  Lane 3: Taq DNA Polymerase + Control antibody

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