Anti-Histone H3 antibody [6-A7]

Anti-Histone H3 antibody [6-A7]

• 概述

• 产品描述The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• 产品名称Anti-Histone H3 antibody [6-A7]
• 分子量17 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • H3 histone family, member A antibody
  • H3/A antibody
  • H31_HUMAN antibody
  more

• 应用

  WB: 1:10,000-1:20,000
  ICC: 1:200

•  

  

  Fig1: Western blot analysis on Hela (1), NIH/3T3 (2), PC12(3) cell lysates using anti- Histone H3 Mouse mAb (Cat. # M1306-4).

   

  

  Fig2: ICC staining Histone H3 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Histone H3 mouse monoclonal antibody.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Histone H3 mouse monoclonal antibody.

   

  

  Fig5: Immunofluorescence analysis of paraffin-embedded rat brain tissue using anti-Histone H3 mouse monoclonal antibody.

   

  

  Fig6: Flow cytometric analysis of Hela cells with Histone H3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.

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