Anti-BLNK antibody [G1-F9]

Anti-BLNK antibody [G1-F9]

• 概述

• 产品描述Cross-linking of the B cell receptor (BCR) activates a variety of signaling pathways involved in processes such as cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in this BCR signal transduction and are thought to play an important role in B cell development. BLNK (for B cell linker protein) is a central linker protein in B cells which has been shown to associate with the effector proteins GRB2, Vav, Nck and PLC γ following activation of the B cell receptor. The two forms of BLNK, pp68 and pp70, arise from alternate splicing of the human BLNK gene transcript. BLNK is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC γ.
• 产品名称Anti-BLNK antibody [G1-F9]
• 分子量70 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosplasm. Cell membrane.
• 其它名称
  • AGM4 antibody
  • B cell adapter containing SH2 domain protein antibody
  • B cell adapter containing Src homology 2 domain protein antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:200
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of BLNK on human BLNK recombinant protein using anti-BLNK antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of BLNK on NIH/3T3 (1) and BCBL-1 (2) cell lysate using anti-BLNK antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining BLNK (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-BLNK antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of NIH/3T3 cells with BLNK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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