Anti-AKAP12 antibody

Anti-AKAP12 antibody

• 概述

• 产品描述Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).
• 产品名称Anti-AKAP12 antibody
• 分子量191 KDa
• 种属反应性Human,Mouse,Rat,Dog
• 验证应用WB,IHC-P,ICC/IF,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human AKAP12 701-900/1684
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm
• 其它名称A kinase (PRKA) anchor protein (gravin) 12
  A kinase Anchor Protein 12
  A kinase anchor protein 250kDa
  AKAP 12
  AKAP 250
  AKAP250
  DKFZp686M0430
  DKFZp686O0331
  Gercelin
  Gravin
  Kinase scaffold protein gravin
  Myasthenia gravis autoantigen gravin
  Srcs5
  SSeCKS
  Tsga12.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1µg/Test
  IF:1:200-800

•  

  

  Fig1: Blank control: RSC 96 (blue).
  Primary Antibody:Rabbit Anti-AKAP12 antibody( Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Antibodywere incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody ofat 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (AKAP12) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig3: Sample:
  Lane 1: mouse bladder Lysate at 25 ug
  Lane 2: mouse lung Lysate at 25 ug
  Primary: Anti-AKAP12at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 191kD
  Observed band size: 191kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！