Anti-TBL1X/SMAP55 antibody
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概述
- 产品描述F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
- 产品名称Anti-TBL1X/SMAP55 antibody
- 分子量63 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human TBL1X 501-577/577
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus (Probable).
- 其它名称TBL1
EBI
F box like/WD repeat protein TBL1X
SMAP 55
SMAP55
TBL 1
TBL1X
Transducin (beta) like 1
Transducin (beta) like 1 X linked
Transducin beta like 1 X linked
Transducin beta like 1X
Transducin beta like 1X protein
TBL1X_HUMAN
F-box-like/WD repeat-containing protein TBL1X
Full=Transducin beta-like protein 1X
Transducin-beta-like protein 1, X-linked. .
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- SMAP55 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TBL1X) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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