Anti-MAPK14 antibody
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概述
- 产品描述Mitogen-activated protein kinase 14, also called p38-α, is an enzyme that in humans is encoded by the MAPK14 gene. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response.
- 产品名称Anti-MAPK14 antibody
- 分子量40 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P
- 抗体类型小鼠单抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2a
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm, nucleus
- 其它名称
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
more
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应用
WB: 1:500
ICC: 1:100-1:200
IHC-P: 1:100-1:200
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Fig1: Western blot analysis of MAPK14 on different cell lysates using anti-MAPK14 antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: HepG2
Lane 3: Hela
Lane 4: NIH/3T3
Lane 5: Jurkat
Lane 6: 293T
Lane 7: mouse placenta
Fig2: ICC staining of MAPK14 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-MAPK14 antibody. Counter stained with hematoxylin.
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