Anti-MUC5AC antibody [G6-H8]

Anti-MUC5AC antibody [G6-H8]

• 概述

• 产品描述Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. Mucin 6 carries GlcNAca1-->4Galb-->R structures, indicating that alpha1, 4-N-acetylglucosaminyltransferase is important to the formation of the mucous glycoproteins in vivo. Mucin 5AC is a gel-forming mucin that is secreted from surface mucous cells. Glucocorticoid is required for the expression of Mucin 5AC mRNA and high doses of hydrocortisone suppress its expression. Additionally, asthmatic fluid stimulates Mucin 5AC synthesis several-fold. The pro-inflammatory cytokines IL-6 and TNF-alpha stimulate Mucin 5AC secretion and thus contribute to the upregulation of mucin by chronic inflammation. Expression of Mucin 5AC is retinoic acid (RA)- or retinol- dependent and RA control of mucin genes is mediated by the retinoid acid receptor RARα and, to a lesser extent, by RARγ. Thyroid hormone binding to thyroid receptors inhibits Mucin 5AC gene expression. Mucin 5AC is also expressed in normal endocervical epithelium, small intestine, gastric cells (Lewis type 1) and gastric metaplasia, and it is a one of the major mucins in the ethmoid mucosa.
• 产品名称Anti-MUC5AC antibody [G6-H8]
• 分子量585 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Secreted, Cytoplasmic, cell surface.
  
• 其它名称
  • Apomucin antibody
  • Apomucin Major Airway Glycoprotein antibody
  • Gastric mucin antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:200-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Mucin 5AC on human Mucin 5AC recombinant protein using anti- Mucin 5AC antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Mucin 5AC on HEK293 (1) and Mucin 5AC -hIgGFc transfected HEK293 (2) cell lysate using anti- Mucin 5AC antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using anti- Mucin 5AC antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human stomach tissues using anti- Mucin 5AC antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with Mucin 5AC antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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